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KMID : 0903620040450060281
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Journal of the Korean Society for Horticultural Science
2004 Volume.45 No. 6 p.281 ~ p.286
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Microtuberization Response in Several Genotypes of Potato (Solanum tuberosum L.) by Direct Addition of Liquid Medium to in vitro Plantlets
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Dhital Shambhu P.
Lim Hak-Tae
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Abstract
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In vitro plantlets of eight potato varieties ‘Superior’, ‘Gogu Valley’, ‘Juice Valley’, ‘Early Valley’, ‘Winter Valley’, ‘Taedong Valley’, ‘Purple Valley’, and ‘Golden Valley’ were used to investigate the effect of an additional Murashige and Skoog (MS) liquid medium supplemented with sucrose and plant growth substances (PGSs) on the production of large size microtubers. Plantlets were grown in MS solid medium containing 6% sucrose and incubated for the first 60 days under long day condition (16 h light/day), and then added with MS liquid medium with 8% sucrose. These plantlets produced the highest numbers (1.7 microtubers/plantlet) and yield (635 ㎎/plantlet) of microtuber. The lowest number and yield were obtained from the in vitro plantlets grown in the solid medium without adding MS liquid medium. As for the utilization of PGSs, it was shown that the higher the PGSs levels, earlier the tuberizations with higher yield and lower number of microtubers. The highest yield (591 ㎎/plantlet) was obtained from 200 ㎎ㆍL?¹ of succinic acid 2,2-dimethylhydrazide (B-9) and numbers (1.6 microtubers/plantlet) from 10 ㎎ㆍL?¹ of 6-benzylaminopurine (BAP) treatment. In the case of combinations of PGSs, treatments of 10 ㎎ㆍL?¹ BAP and 200 ㎎ㆍL?¹ B-9 produced significantly the highest numbers (1.5/plantlet) and the highest yield (766 ㎎/plantlet) of microtuber, but lowest from the control. Thus, an extra addition of MS liquid medium with sucrose and PGSs might have played a major role for the further induction and development of large size of microtubers on diverse genetic background of potatoes under in vitro conditions.
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KEYWORD
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6-benzylaminopurine, microtuber, plant growth substances, succinic acid 2, 2-dimethylhydrazide
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